Molecular responses as indicators of marine pollution: DNA damage and enzyme induction in Limanda limanda and Asterias rubens.
نویسندگان
چکیده
During a survey from 26 August through 13 September 1991, specimens of the flatfish, Limanda limanda (dab), and the asteroid echinoderm Asterias rubens (seastar), were collected at sampling locations along transects radiating into the North Sea from the coastal zone of The Netherlands. In homogenates of liver tissue from male dab and the digestive gland (pyloric caeca) of female seastar, DNA damage (strand breaks) and induction of the cytochrome P450-dependent monooxygenase system (MO) were determined. Areas could be described with significantly increased percentages of strand breaks (lower integrity) both in dab and seastar. However, enhanced DNA strand breaks did not correspond with contamination gradients, expressed as concentrations of polychlorinated biphenyls (PCBs) or polyaromatic hydrocarbons. MO enzyme induction in the hepatic 13,000g fraction of male dab, measured as 7-ethoxyresorufin-O-deethylase activity, was significantly enhanced in response to low ambient temperatures. Some evidence was found for the facilitation of benzo[a]pyrene hydroxylase activity expressing the enzyme induction in the microsomal fraction of pyloric caeca of seastars, at increasing PCB concentrations. DNA integrity and enzyme induction elucidate the physiologic status and might be indicative for ambient impairment within restricted areas, and not necessarily related to the presence of anthropogenic or xenobiotic substances.
منابع مشابه
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas.
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from ...
متن کاملSpatial distribution of polyfluoroalkyl compounds in dab (Limanda limanda) bile fluids from Iceland and the North Sea.
The spatial distribution of polyfluoroalkyl compounds (PFCs) was investigated in dab (Limanda limanda) bile fluids collected from Iceland and the North Sea. Concentrations of various PFCs, including perfluorinated sulfonates (C(4)-C(6), C(8) PFSAs), perfluorinated carboxylic acids (C(9)-C(14) PFCAs) and n-methyl perfluorooctane sulfonamidoethanol (MeFOSE), were quantified. Perfluorooctane sulfo...
متن کاملSalinity gradient shapes distance decay of similarity among parasite communities in three marine fishes.
Published data were used to compare the distance decay of similarity in parasite communities of three marine fish hosts: Atlantic cod Gadus morhua, the dab Limanda limanda and the flounder Platichthys flesus in two adjacent areas that differ with respect to the strength of a salinity gradient. In the Baltic Sea, which exhibits a strong salinity gradient from its connection with the North Sea in...
متن کاملComparative nutritional condition of larval dab Limanda limanda and lesser sandeel Ammodytes marinus in a highly variable environment
We investigated the nutritional condition of larval fishes caught in daily ichthyoplankton hauls in the ‘Helgoland Roads’ (54° 11.18’ N and 07° 54.00’ E) from February to June 2004. We concentrated on larvae of dab Limanda limanda and lesser sandeel Ammodytes marinus in order to compare early life stages of iteroparous and nearly semelparous fish. We analysed length, weight and RNA:DNA ratios a...
متن کاملQuantitative assessment of melanomacrophage centres (MMCs) in dab Limanda limanda along a pollution transect in the German Bight
This study attempted to demonstrate whether an aspect of non-specd~c ellular immunity could be used as an lnd~cator of f ~ s h health In populatlons of dab Lllnanda l imanda exposed along a pollut~on gradient Replicate samples of dab spleens were ob ta~ned durlng the Bremerhaven Workshop from a senes of stations along a transect in the German B ~ g h t H~stochem~cal techn~ques , to demonstrate ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Environmental Health Perspectives
دوره 102 شماره
صفحات -
تاریخ انتشار 1994